The European Medicines Agency, the European Centre for Disease Prevention and Control, the European Chemicals Agency, the European Environment Agency and the European Food Safety Authority have jointly published a framework for action to enhance cooperation to support the implementation of the One Health agenda in the EU. One Health identifies the complex interplay between human, animal and plant health, food safety, the climate crisis and environmental sustainability. Implementation of this approach across different sectors will help the EU to be better equipped to predict, prevent, detect and respond to health threats. A cross-agency task force will work on implementing the joint framework for action from 2024–26, which will focus on five strategic objectives—strategic coordination, research coordination, capacity building, stakeholder engagement and joint inter-agency activities. This will integrate the scientific advice provided by the agencies and enhance the evidence base for One Health.